<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2025 Workiva-->
<!--r:1bedb9dd-fd8a-4562-ae0b-6d7748239304,g:11c3436d-3d3d-4b98-9d8f-e60b64825a62-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.genedx.com/role/DocumentandEntityInformationDocument" xlink:type="simple" xlink:href="wgs-20250415.xsd#DocumentandEntityInformationDocument"/>
  <link:definitionLink xlink:role="http://www.genedx.com/role/DocumentandEntityInformationDocument" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntitiesTable_b9dbbbd5-2230-44bd-8408-88de7ca43960" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntitiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_EntitiesTable_b9dbbbd5-2230-44bd-8408-88de7ca43960" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_c4079069-74aa-4ef9-aee7-cc68ae489b5b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_dei_EntitiesTable_b9dbbbd5-2230-44bd-8408-88de7ca43960" xlink:to="loc_us-gaap_StatementClassOfStockAxis_c4079069-74aa-4ef9-aee7-cc68ae489b5b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_c4079069-74aa-4ef9-aee7-cc68ae489b5b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_c4079069-74aa-4ef9-aee7-cc68ae489b5b" xlink:to="loc_us-gaap_ClassOfStockDomain_c4079069-74aa-4ef9-aee7-cc68ae489b5b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_977783f8-6798-429c-bf7a-0f89e29bc633" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_c4079069-74aa-4ef9-aee7-cc68ae489b5b" xlink:to="loc_us-gaap_ClassOfStockDomain_977783f8-6798-429c-bf7a-0f89e29bc633" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_63bc498e-f1f8-4908-b3ad-9e87b2609de5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_977783f8-6798-429c-bf7a-0f89e29bc633" xlink:to="loc_us-gaap_CommonClassAMember_63bc498e-f1f8-4908-b3ad-9e87b2609de5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WarrantMember_13613b79-10b9-46ce-af44-d4edcd3ddb7e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_WarrantMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_977783f8-6798-429c-bf7a-0f89e29bc633" xlink:to="loc_us-gaap_WarrantMember_13613b79-10b9-46ce-af44-d4edcd3ddb7e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_cca97211-14ce-476b-9e58-e4c26a6ff066" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_DocumentType"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_DocumentType_cca97211-14ce-476b-9e58-e4c26a6ff066" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_e198ae86-4cf6-4bc7-b313-3b4e51aeb2eb" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_DocumentPeriodEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_DocumentPeriodEndDate_e198ae86-4cf6-4bc7-b313-3b4e51aeb2eb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_0c05e3b2-dafe-488a-9529-69d3acc41354" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityRegistrantName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_EntityRegistrantName_0c05e3b2-dafe-488a-9529-69d3acc41354" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_86de5d0c-1228-4d21-8bca-ec5478b5d1f7" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_EntityIncorporationStateCountryCode_86de5d0c-1228-4d21-8bca-ec5478b5d1f7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_0aa16a75-ca39-4e21-8767-2f115f385f34" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityFileNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_EntityFileNumber_0aa16a75-ca39-4e21-8767-2f115f385f34" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_0ebe0f53-f828-45b2-ada8-171631acead3" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_EntityTaxIdentificationNumber_0ebe0f53-f828-45b2-ada8-171631acead3" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_8f278e9f-9e51-4eda-9199-d39cc70a0041" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressAddressLine1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_EntityAddressAddressLine1_8f278e9f-9e51-4eda-9199-d39cc70a0041" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_9eb9e87c-1732-41f2-9ef8-b8be97de5551" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressCityOrTown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_EntityAddressCityOrTown_9eb9e87c-1732-41f2-9ef8-b8be97de5551" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_cc4fdff3-de85-465b-9d35-844b118f87b6" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressStateOrProvince"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_EntityAddressStateOrProvince_cc4fdff3-de85-465b-9d35-844b118f87b6" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_f59823ab-2608-4513-957b-5f6e52c0a783" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressPostalZipCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_EntityAddressPostalZipCode_f59823ab-2608-4513-957b-5f6e52c0a783" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_1824b189-d66e-4a63-8861-bb9bb2c848d6" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_CityAreaCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_CityAreaCode_1824b189-d66e-4a63-8861-bb9bb2c848d6" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_3968382a-9ca9-40ef-a6ce-ab4190ee7bb5" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LocalPhoneNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_LocalPhoneNumber_3968382a-9ca9-40ef-a6ce-ab4190ee7bb5" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_WrittenCommunications_098def07-585f-49ae-aab8-8f28adb76335" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_WrittenCommunications"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_WrittenCommunications_098def07-585f-49ae-aab8-8f28adb76335" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SolicitingMaterial_24d0f7ae-a099-42f1-be68-67d2543f6a22" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_SolicitingMaterial"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_SolicitingMaterial_24d0f7ae-a099-42f1-be68-67d2543f6a22" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_PreCommencementTenderOffer_1577c10b-fa23-4c9a-bd26-13f0e8acddcb" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_PreCommencementTenderOffer"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_PreCommencementTenderOffer_1577c10b-fa23-4c9a-bd26-13f0e8acddcb" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_PreCommencementIssuerTenderOffer_ac569782-87b8-4055-8cb9-ac8d360ee131" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_PreCommencementIssuerTenderOffer"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_PreCommencementIssuerTenderOffer_ac569782-87b8-4055-8cb9-ac8d360ee131" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_f8e1b4a1-1993-4a65-9ce6-0379de17bb29" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_Security12bTitle"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_Security12bTitle_f8e1b4a1-1993-4a65-9ce6-0379de17bb29" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_befdb413-3648-4618-9b52-fb1fa26f08db" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_TradingSymbol"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_TradingSymbol_befdb413-3648-4618-9b52-fb1fa26f08db" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_dfe66e69-3756-488d-8604-a90cc0cf7d9a" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_SecurityExchangeName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_SecurityExchangeName_dfe66e69-3756-488d-8604-a90cc0cf7d9a" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_8bc8da31-2163-47ba-a609-89c7efe0d9c8" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_EntityEmergingGrowthCompany_8bc8da31-2163-47ba-a609-89c7efe0d9c8" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityExTransitionPeriod_22950231-a3c3-45a4-b36a-9ebe9c4edc6e" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityExTransitionPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_EntityExTransitionPeriod_22950231-a3c3-45a4-b36a-9ebe9c4edc6e" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine2_623891c1-76f2-4b1a-8b3d-5219a63f81b4" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressAddressLine2"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_EntityAddressAddressLine2_623891c1-76f2-4b1a-8b3d-5219a63f81b4" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine3_6ad48bdc-a487-4e9c-9398-b11ab8decddc" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressAddressLine3"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_EntityAddressAddressLine3_6ad48bdc-a487-4e9c-9398-b11ab8decddc" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_e9a6c946-1d5f-4c7f-a351-0ac3daa4d6d0" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityCentralIndexKey"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_EntityCentralIndexKey_e9a6c946-1d5f-4c7f-a351-0ac3daa4d6d0" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_cd779cfb-c4ed-42b7-a381-c8c0af3a81e3" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_AmendmentFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_eed892fe-9541-4551-8584-ab2f1e05120c" xlink:to="loc_dei_AmendmentFlag_cd779cfb-c4ed-42b7-a381-c8c0af3a81e3" xlink:type="arc" order="24"/>
  </link:definitionLink>
</link:linkbase>
