United States securities and exchange commission logo May 5, 2022 Daniel Clark General Counsel Sema4 Holdings Corp. 333 Ludlow Street, North Tower, 8th Floor Stamford, Connecticut 06902 Re: Sema4 Holdings Corp. Registration Statement on Form S-1 Filed May 3, 2022 File No. 333-264626 Dear Mr. Clark: This is to advise you that we have not reviewed and will not review your registration statement. Please refer to Rules 460 and 461 regarding requests for acceleration. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Please contact Jessica Ansart at 202-551-4511 with any questions. Sincerely, Division of Corporation Finance Office of Life Sciences cc: Ethan Skerry